A new, simple, precise and accurate method for the estimation of methylcobalamin in bulk and multicomponent pharmaceutical dosage form has been developed. A methanol was chosen as the solvent system. The λmax was found to be 360.0nm. The responses were linear in the range of 2-10μg/ml. The regression equation of the calibration graph and correlation coefficient were found to be y = 0.015x + 0.001 and 0.999 respectively. Validation of the method was done in order to demonstrate accuracy, precision, interday, intraday and assay, of the proposed method. The %RSD values for both intraday and interday precision were less than 2%. The recovery of the drug from the sample was ranged between 98% and 102.0%. multicomponent tablets containing 500mcg of methylcobalamin were analyzed by the proposed method and the results were well within the claimed limits.
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